Scheffer Jan, Tudzynski Paul
Institut für Botanik, Westfälische Wilhelms-Universität, Schlossgarten 3, D-48149 Münster, Germany.
Mycol Res. 2006 Apr;110(Pt 4):465-70. doi: 10.1016/j.mycres.2005.11.011. Epub 2006 Feb 14.
The pathogenic development of the biotrophic ergot fungus Claviceps purpurea is strictly limited to the ovary of grasses. Early colonization stages occur within a defined spatio-temporal course of events, including the directed growth to the vascular tissue for nutrient supply. To characterize mutant strains with putative defects in pathogenicity, the close observation of the infection pathway is therefore indispensable. Here, we describe the establishment of a new pathogenicity assay, based on the in vitro cultivation of isolated rye ovaries. The pathogenic development of a wild-type strain of C. purpurea was compared with the infection of mature rye flowers on whole plants. Up to the sixth day post inoculation, the route of infection within the isolated ovaries was maintained and temporally equal to that seen in mature flowers. Therefore, the in vitro pathogenicity assay is an effective alternative to the whole-plant infection tests, and suitable for detailed infection studies and screening high numbers of mutants for defects in early pathogenesis.
专性寄生麦角菌紫麦角菌的致病发育严格局限于禾本科植物的子房。早期定殖阶段发生在特定的时空事件进程中，包括向维管组织定向生长以获取营养供应。因此，为了鉴定具有假定致病性缺陷的突变菌株，密切观察感染途径是必不可少的。在此，我们描述了一种基于离体黑麦子房体外培养的新致病性测定方法的建立。将紫麦角菌野生型菌株的致病发育与整株植物上成熟黑麦花的感染情况进行了比较。接种后第六天，离体子房内的感染途径得以维持，且在时间上与成熟花中的情况相同。因此，体外致病性测定是全株感染试验的有效替代方法，适用于详细的感染研究以及筛选大量早期发病机制存在缺陷的突变体。
