Schoenfisch Mark H, Rothrock Aaron R, Shin Jae Ho, Polizzi Mark A, Brinkley Michael F, Dobmeier Kevin P
Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3290, USA.
Biosens Bioelectron. 2006 Aug 15;22(2):306-12. doi: 10.1016/j.bios.2006.01.012. Epub 2006 Feb 17.
Nitric oxide (NO)-releasing xerogel membranes were prepared as coatings for an electrochemical glucose biosensor to allow for enhanced biocompatibility while maintaining adequate response times and sensitivity. Formation of the NO-donor species was found to drastically decrease the permeability of the aminosilane-based xerogels to both hydrogen peroxide and glucose. The addition of poly(vinylpyrrolidone) (PVP) polymer enhanced the membrane permeability even after exposure to high pressures of NO (necessary for NO-donor synthesis). The analytical response and NO release of PVP-doped NO-releasing xerogels as glucose sensor membranes were further investigated and found to be enhanced via polymer doping. Doping of the polymer into the xerogel did not compromise the stability of the xerogel as evaluated by silicon leaching studies. Despite the addition of PVP, the NO-releasing xerogels maintained reduced bacterial adhesion characteristics analogous to previous reports for NO-releasing xerogels.
制备了释放一氧化氮（NO）的干凝胶膜作为电化学葡萄糖生物传感器的涂层，以在保持足够响应时间和灵敏度的同时提高生物相容性。发现NO供体物种的形成会大幅降低基于氨基硅烷的干凝胶对过氧化氢和葡萄糖的渗透性。即使在暴露于高浓度NO（NO供体合成所必需）之后，添加聚乙烯吡咯烷酮（PVP）聚合物也能提高膜的渗透性。进一步研究了掺杂PVP的释放NO的干凝胶作为葡萄糖传感器膜的分析响应和NO释放情况，发现通过聚合物掺杂可增强这些性能。通过硅浸出研究评估，将聚合物掺杂到干凝胶中不会损害干凝胶的稳定性。尽管添加了PVP，但释放NO的干凝胶仍保持了与先前关于释放NO的干凝胶报道类似的降低细菌粘附特性。
