血影蛋白与脂肪酸疏水相互作用的电子顺磁共振研究
EPR study of the hydrophobic interaction of spectrin with fatty acids.
作者信息
Streichman S, Kahana E, Silver B L
机构信息
Department of Hematology, Rambam Medical Center, Haifa, Israel.
出版信息
Biochim Biophys Acta. 1991 Jul 1;1066(1):9-13. doi: 10.1016/0005-2736(91)90243-2.
PMID:1648396
Abstract
The hydrophobic interaction between spin-labelled stearic acid and spectrin was studied by electron paramagnetic resonance (EPR) and fluorescence quenching. The results are quantitatively interpreted in terms of two types of binding site on spectrin. A comparison between the results of the EPR and fluorescence experiments show the drawback of the fluorescence method in binding studies.
摘要
通过电子顺磁共振（EPR）和荧光猝灭研究了自旋标记硬脂酸与血影蛋白之间的疏水相互作用。根据血影蛋白上两种类型的结合位点对结果进行了定量解释。EPR实验结果与荧光实验结果的比较显示了荧光法在结合研究中的缺点。
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