Hainsworth P J, Raphael K L, Stillwell R G, Bennett R C, Garson O M
Department of Surgery, University of Melbourne, Australia.
Cancer Genet Cytogenet. 1991 Jun;53(2):205-18. doi: 10.1016/0165-4608(91)90097-e.
Tumor preparations from 26 primary breast cancers were studied cytogenetically with G-banding, using a direct technique, synchronized short-term culture, or both. Two tumors had normal karyotypes, and 24 (92%) had chromosomal abnormalities. Nineteen tumors had chromosome 1 rearrangements, with 10 cases (40%) displaying distal short arm translocations (1p36). Other frequent breakpoints occurred at 3p21, 6q22-27, 11q21-25, 16q22-24, 17p, and 19q13. To seek primary rather than secondary cytogenetic changes, attention was directed toward tumors with diploid-range karyotypes (32-57 chromosomes per cell). Of four such tumors, three exhibited nonrandom involvement of chromosome 16q22. This, together with previously reported data, suggests that deletion or rearrangement of chromosome 16q21-24 may be a primary or specific event in a subset of breast cancers.
采用直接技术、同步短期培养或两者结合的方法，运用G显带技术对26例原发性乳腺癌的肿瘤标本进行了细胞遗传学研究。2例肿瘤核型正常，24例（92%）存在染色体异常。19例肿瘤有1号染色体重排，其中10例（40%）表现为短臂远端易位（1p36）。其他常见的断点位于3p21、6q22 - 27、11q21 - 25、16q22 - 24、17p和19q13。为了寻找原发性而非继发性细胞遗传学改变，研究重点放在了核型处于二倍体范围（每个细胞32 - 57条染色体）的肿瘤上。在4例这样的肿瘤中，3例表现出16q22的非随机受累。这与先前报道的数据一起表明，16q21 - 24染色体的缺失或重排在一部分乳腺癌中可能是一个原发性或特异性事件。
