Giraldez Antonio J, Mishima Yuichiro, Rihel Jason, Grocock Russell J, Van Dongen Stijn, Inoue Kunio, Enright Anton J, Schier Alexander F
Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, and Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA.
Science. 2006 Apr 7;312(5770):75-9. doi: 10.1126/science.1122689. Epub 2006 Feb 16.
MicroRNAs (miRNAs) comprise 1 to 3% of all vertebrate genes, but their in vivo functions and mechanisms of action remain largely unknown. Zebrafish miR-430 is expressed at the onset of zygotic transcription and regulates morphogenesis during early development. By using a microarray approach and in vivo target validation, we find that miR-430 directly regulates several hundred target messenger RNA molecules (mRNAs). Most targets are maternally expressed mRNAs that accumulate in the absence of miR-430. We also show that miR-430 accelerates the deadenylation of target mRNAs. These results suggest that miR-430 facilitates the deadenylation and clearance of maternal mRNAs during early embryogenesis.
微小RNA（miRNA）占所有脊椎动物基因的1%至3%，但其在体内的功能和作用机制仍 largely未知。斑马鱼miR-430在合子转录开始时表达，并在早期发育过程中调节形态发生。通过使用微阵列方法和体内靶点验证，我们发现miR-430直接调节数百个靶信使RNA分子（mRNA）。大多数靶点是母源表达的mRNA，在没有miR-430的情况下积累。我们还表明，miR-430加速了靶mRNA的去腺苷酸化。这些结果表明，miR-430在早期胚胎发育过程中促进了母源mRNA的去腺苷酸化和清除。
