Claman L J, Stetson D, Steinberg B, Shuler C F
Department of Periodontology, Ohio State University, Columbus.
J Oral Pathol Med. 1991 May;20(5):245-9. doi: 10.1111/j.1600-0714.1991.tb00428.x.
Thin section and freeze-fracture transmission electron microscopy were used to examine and identify the cytoplasmic and membrane structures in a cell line derived from a melanotic neuroectodermal tumor of infancy (MNTI). The cultured cells had a uniform appearance after 70 population doublings characterized by long dendritic processes and evidence of melanin production. The cytoplasm contained numerous melanosomes in various stages of development, vesiculated rough endoplasmic reticulum, microfilaments and uncoated as well as coated vesicles. The membrane specializations included caveoli, coated pits, gap junctions, microfilaments, desmosome-like structures and lamellipodia. The ultrastructural appearance of the cultured MNTI cells was similar to features previously seen in electron micrographs of MNTI tumor specimens. However, correlated freeze-fracture and thin section micrographs permitted further identification of structures previously described. The MNTI cell line represents one of the cell types of the tumor and provides an opportunity for further study of the pathogenesis of this rare tumor.
采用超薄切片和冷冻断裂透射电子显微镜技术，对源自婴儿黑色素性神经外胚层肿瘤（MNTI）的细胞系中的细胞质和膜结构进行检查和鉴定。经过70次群体倍增后，培养的细胞具有统一的外观，其特征为长的树突状突起和黑色素生成的证据。细胞质中含有许多处于不同发育阶段的黑素小体、泡状粗面内质网、微丝以及无包被和有包被的小泡。膜特化结构包括小窝、有被小窝、缝隙连接、微丝、桥粒样结构和片状伪足。培养的MNTI细胞的超微结构外观与先前在MNTI肿瘤标本的电子显微照片中所见特征相似。然而，相关的冷冻断裂和超薄切片显微照片能够进一步鉴定先前描述的结构。MNTI细胞系代表了肿瘤的细胞类型之一，为进一步研究这种罕见肿瘤的发病机制提供了机会。
