Levy M S, Pomposiello P, Nagel R
Cefybo, Buenos Aires, Argentina.
Mutat Res. 1991 Jul;255(1):95-100. doi: 10.1016/0921-8777(91)90022-h.
UV irradiation induced the precise excision of Tn10 inserted in met, trp or srl in a Salmonella typhimurium strain; mitomycin C was also found to induce the frequency of precise excision of Tn10 from srl or met. Precise excision of Tn10 was not increased by either UV or mitomycin C in a recA mutant. Similarly, a recA mutant derived from a uvrD strain showed a drastic reduction in the high spontaneous levels of precise excision of Tn10 of this strain. These results indicate that recA is involved in the increased precise excision of Tn10. In contrast to point mutations excision of Tn10 was found to be UV inducible in a top mutant.
紫外线照射可诱导鼠伤寒沙门氏菌菌株中插入met、trp或srl的Tn10精确切除；还发现丝裂霉素C可诱导Tn10从srl或met精确切除的频率增加。在recA突变体中，紫外线或丝裂霉素C均未增加Tn10的精确切除率。同样，源自uvrD菌株的recA突变体显示，该菌株Tn10高自发精确切除水平大幅降低。这些结果表明，recA参与了Tn10精确切除率的增加。与点突变相反，发现Tn10的切除在top突变体中是紫外线可诱导的。
