Hafner L M, MacPhee D G
Department of Microbiology, La Trobe University, Bundoora, Victoria, Australia.
Mutat Res. 1990 Oct;245(2):125-8. doi: 10.1016/0165-7992(90)90011-8.
Precise excision of transposon Tn10, as judged by reversion of Salmonella typhimurium strain LT2 trp1014::Tn10 to Trp+, was not detectably enhanced following exposure to 9-aminoacridine, 5-azacytidine or mitomycin C in conventional treat-and-plate assays. By contrast, 7/13 chemicals, including 5-azacytidine and mitomycin C, were found to be capable of enhancing precise excision of Tn10 when tested in modified fluctuation assays. Despite earlier reports, precise excision is one activity of transposons which is not therefore refractory to enhancement by chemical mutagens.
通过鼠伤寒沙门氏菌LT2 trp1014::Tn10回复为Trp +来判断，在传统的处理 - 铺板试验中，暴露于9 - 氨基吖啶、5 - 氮杂胞苷或丝裂霉素C后，转座子Tn10的精确切除没有明显增强。相比之下，在改良的波动试验中进行测试时，发现包括5 - 氮杂胞苷和丝裂霉素C在内的13种化学物质中的7种能够增强Tn10的精确切除。尽管有早期报道，但精确切除是转座子的一种活性，因此并非对化学诱变剂的增强作用具有抗性。
