Cai S, Shimizu T, Tomioka H
Department of Microbiology and Immunology, Shimane University School of Medicine, Izumo, Shimane 693-8501, Japan.
Clin Exp Immunol. 2006 Mar;143(3):560-71. doi: 10.1111/j.1365-2249.2006.03016.x.
Mycobacterium avium complex-induced immunosuppressive macrophages (MAC-MPhis) exhibit suppressor activity against concanavalin A-induced T cell mitogenesis (T cell Con A mitogenesis). We examined the profiles of the MAC-MPhi-mediated suppression of lipopolysaccharide-induced B cell mitogenesis (B cell LPS mitogenesis) and found the following. First, although N(G)-monomethyl-L-arginine and carboxy-PTIO effectively blocked the MAC-MPhi's suppressor activity against T cell Con A mitogenesis, MAC-MPhi's action against B cell LPS mitogenesis was only weakly affected by these NO-reducing agents. Second, B cell LPS mitogenesis was remarkably more susceptible to MAC-MPhi-derived reactive oxygen intermediates than T cell Con A mitogenesis. Third, B cell LPS mitogenesis was less susceptible to the inhibitory effects of the other MAC-MPhi-derived suppressor mediators, including free fatty acids, TGF-beta and prostaglandin E(2), than T cell Con A mitogenesis. Fourth, MAC-MPhi's suppressor activity was strongly dependent on B7-1 like molecule-mediated cell contact with target cells only in the case of T cell Con A mitogenesis. Therefore, there are significant differences in the modes of suppressor action of MAC-MPhis against T cell and B cell mitogenesis.
鸟分枝杆菌复合群诱导的免疫抑制巨噬细胞（MAC - MPhis）对伴刀豆球蛋白A诱导的T细胞有丝分裂（T细胞伴刀豆球蛋白A有丝分裂）表现出抑制活性。我们研究了MAC - MPhi介导的对脂多糖诱导的B细胞有丝分裂（B细胞脂多糖有丝分裂）的抑制情况，结果如下。首先，尽管N(G)-单甲基-L-精氨酸和羧基-PTIO有效地阻断了MAC - MPhi对T细胞伴刀豆球蛋白A有丝分裂的抑制活性，但这些一氧化氮还原剂对MAC - MPhi对B细胞脂多糖有丝分裂的作用影响较弱。其次，B细胞脂多糖有丝分裂比T细胞伴刀豆球蛋白A有丝分裂对MAC - MPhi衍生的活性氧中间体更敏感。第三，B细胞脂多糖有丝分裂比T细胞伴刀豆球蛋白A有丝分裂对其他MAC - MPhi衍生的抑制介质（包括游离脂肪酸、转化生长因子-β和前列腺素E(2)）的抑制作用更不敏感。第四，仅在T细胞伴刀豆球蛋白A有丝分裂的情况下，MAC - MPhi的抑制活性强烈依赖于B7 - 1样分子介导的与靶细胞的细胞接触。因此，MAC - MPhis对T细胞和B细胞有丝分裂的抑制作用模式存在显著差异。
