Ramirez de Knott Haydee M, McCormick Thomas S, Kalka Katrin, Skandamis George, Ghannoum Mahmoud A, Schluchter Mark, Cooper Kevin D, Nedorost Susan T
Department of Dermatology, Case Western Reserve University and University Hospitals of Cleveland, OH 44106, USA.
Contact Dermatitis. 2006 Feb;54(2):92-9. doi: 10.1111/j.0105-1873.2006.00774.x.
Atopic dermatitis (AD) patients with predominantly head and neck involvement react to patch tests of the yeast Malassezia sympodialis (Ms). Protein patch testing methods and interpretation are controversial, but subgroups of AD patients may have unique triggers for disease activity. The aim of the study was to identify clinical characteristics of patients who are patch test-positive to Dermatophagoides farinae/pteronyssinus (Df) and Ms and characterize cutaneous cytokine profiles of the atopy patch tests (APTs). 25 AD patients and 27 control dermatitis patients were patch tested with Ms and Df. Qualitative analysis of Th-1 and Th-2 cytokines by RT-PCR mRNA was obtained from positive APTs. Atopic dermatitis patients with a textile pattern or head and neck involvement demonstrated more positive APTs to Ms than control patients. Early positive APTs (<6 hr) did not exhibit a Th-1 type cytokine profile. The subgroup of adult AD patients with head, neck and upper torso pattern of dermatitis seems most likely to react to Ms (and Df). The immune mechanism of protein patch tests includes a Th-1 cell-mediated component after 6 hr or more.
以头颈部受累为主的特应性皮炎（AD）患者对酵母合轴马拉色菌（Ms）的斑贴试验有反应。蛋白质斑贴试验方法及解读存在争议，但AD患者亚组可能有引发疾病活动的独特触发因素。本研究的目的是确定对粉尘螨/屋尘螨（Df）和Ms斑贴试验呈阳性的患者的临床特征，并描述特应性斑贴试验（APT）的皮肤细胞因子谱。对25例AD患者和27例对照性皮炎患者进行了Ms和Df的斑贴试验。通过RT-PCR mRNA对Th-1和Th-2细胞因子进行定性分析，取材于阳性的APT。有皮肤纹理或头颈部受累的特应性皮炎患者对Ms的APT阳性率高于对照患者。早期阳性的APT（<6小时）未表现出Th-1型细胞因子谱。患有头颈部和上躯干性皮炎模式的成年AD患者亚组似乎最有可能对Ms（和Df）产生反应。蛋白质斑贴试验的免疫机制包括6小时或更长时间后由Th-1细胞介导的成分。
