用于快速筛查绵羊李斯特菌病的诊断工具组合。

A combination of diagnostic tools for rapid screening of ovine listeriosis.

作者信息

Amagliani G, Giammarini C, Omiccioli E, Merati E G, Pezzotti G, Filippini G, Brandi G, Magnani M

机构信息

Centro di Biotecnologie, Università degli Studi di Urbino, via T. Campanella 1, 61032 Fano, PU, Italy.

出版信息

Res Vet Sci. 2006 Oct;81(2):185-9. doi: 10.1016/j.rvsc.2005.12.006. Epub 2006 Feb 17.

DOI:10.1016/j.rvsc.2005.12.006

PMID:16487553

Abstract

A combined serological and PCR method for the detection of Listeria monocytogenes infection in symptomatic and asymptomatic ovine flocks was evaluated. Seventy-eight milk samples and 157 serum samples were analysed using a L. monocytogenes PCR detection kit and an anti-listeriolysin O IgG immunoassay kit. The combined use of these commercial kits allowed a rapid and effective detection of L. monocytogenes infection in both the early stage, before seroconversion, and in a later phase, even after antibiotic therapy.

摘要

对一种用于检测有症状和无症状绵羊群中单核细胞增生李斯特菌感染的血清学和PCR联合方法进行了评估。使用单核细胞增生李斯特菌PCR检测试剂盒和抗李斯特菌溶血素O IgG免疫分析试剂盒对78份牛奶样本和157份血清样本进行了分析。这些商业试剂盒的联合使用能够在血清转化前的早期阶段以及甚至在抗生素治疗后的后期阶段快速有效地检测单核细胞增生李斯特菌感染。