McInnes L M, Irwin P, Palmer D G, Ryan U M
Division of Health Sciences, School of Veterinary and Biomedical Science, Murdoch University, WA 6150, Australia.
Vet Parasitol. 2006 Apr 30;137(3-4):355-63. doi: 10.1016/j.vetpar.2006.01.018. Epub 2006 Feb 17.
Neospora caninum was isolated and established in vitro from the skin lesion of a naturally infected dog. The identity of the parasite was evaluated by immunofluorescent antibody test (IFAT), microscopy, Western blotting and polymerase chain reaction (PCR). N. caninum DNA was detected in the whole blood, serum, skin lesion, rectal scrapings and faeces of the infected dog utilising a nested PCR targeting the Nc-5 gene of N. caninum. Antigenic and genetic characterisation of the isolate, designated WA-K9, at a number of loci including the Nc-5 gene, heat shock protein 70 (HSP-70) gene, alpha-tubulin and beta-tubulin genes revealed no variation between this isolate and two N. caninum isolates from different geographic areas. Clinical aspects of this case, which included cutaneous and neurological disease, are also discussed.
从一只自然感染犬的皮肤损伤中分离出犬新孢子虫并在体外进行培养。通过免疫荧光抗体试验（IFAT）、显微镜检查、蛋白质印迹法和聚合酶链反应（PCR）对该寄生虫进行鉴定。利用针对犬新孢子虫Nc-5基因的巢式PCR，在感染犬的全血、血清、皮肤损伤、直肠刮取物和粪便中检测到犬新孢子虫DNA。对命名为WA-K9的分离株在包括Nc-5基因、热休克蛋白70（HSP-70）基因、α-微管蛋白和β-微管蛋白基因等多个位点进行抗原和基因特征分析，结果显示该分离株与来自不同地理区域的两株犬新孢子虫分离株之间没有差异。还讨论了该病例的临床情况，包括皮肤和神经疾病。
