Razmi Gholamreza
Section of Parasitology, Department of Pathobiology, Faculty of Veterinary Medicine, and Excellence Research Center for Ruminantal Abortion and Neonatal Mortality, Ferdowsi University of Mashhad, Mashhad, Iran.
Korean J Parasitol. 2009 Dec;47(4):417-20. doi: 10.3347/kjp.2009.47.4.417. Epub 2009 Dec 2.
Neospora caninum is an important cause of abortion in dairy cattle worldwide. Dog is the definitive host for N. caninum and can infect dairy cattle. The aim of this study is to determine the prevalence of Neospora oocysts in feces of dogs from dairy farms. A total of 174 fecal samples was collected from 89 farm dogs and 85 household dogs during 2006 and 2008. Fecal samples of dogs were microscopically examined for detecting Hammondia Neospora-like oocysts (HNLO) by Mini Parasep SF fecal parasite concentrator. HNLO were microscopically detected in 4 fecal samples (2.2%). The fecal samples with HNLO were examined by N. caninum-specific PCR. Two of the samples were positive for N. caninum. The 2 positive fecal samples were selected for inoculation to calves. Two inoculated calves were seronegative by ELISA for 4 months post-infection. This is the first report of finding N. caninum DNA in feces of farm dogs in Mashhad area, Iran.
犬新孢子虫是全球奶牛流产的一个重要病因。犬是犬新孢子虫的终末宿主,可感染奶牛。本研究的目的是确定奶牛场犬粪便中新孢子虫卵囊的流行情况。2006年至2008年期间,共从89只农场犬和85只家养犬采集了174份粪便样本。通过Mini Parasep SF粪便寄生虫浓缩器对犬的粪便样本进行显微镜检查,以检测哈蒙德新孢子虫样卵囊(HNLO)。在4份粪便样本(2.2%)中显微镜检测到HNLO。对含有HNLO的粪便样本进行犬新孢子虫特异性PCR检测。其中两份样本犬新孢子虫呈阳性。选择这两份阳性粪便样本接种犊牛。两只接种犊牛在感染后4个月ELISA检测血清阴性。这是在伊朗马什哈德地区农场犬粪便中发现犬新孢子虫DNA的首次报告。