Irdani Tiziana, Carletti Beatrice, Ambrogioni Laura, Roversi Pio Federico
CRA-Istituto Sperimentale per la Zoologia Agraria, Cascine del Riecio, Firenze, Italy.
Cryobiology. 2006 Jun;52(3):319-22. doi: 10.1016/j.cryobiol.2006.01.001.
Low temperatures can assure the long-term or even indefinite preservation of important biological specimens. Nematode cryopreservation allows for the availability of large numbers of living nematodes at any one time, especially for experimental purposes. New isolates of Bursaphelenchus have recently been collected, including Bursaphelenchus eremus (Rühm) Goodey. This species was identified in north-central Italy on dying oak trees and from the bark beetle Scolytus intricatus Ratzeburg as dauer larvae. We therefore, sought to develop a cryopreservation technique for the long-term storage of all available Bursaphelenchus spp. The technique consists of a rapid-cooling protocol involving immersion in a liquid nitrogen bath before storage of the frozen samples in a mechanical freezer at -140 degrees C. The survival of nematodes subjected to this rapid-cooling protocol was higher than previously reported using slow-cooling methods and is suitable for several species of Bursaphelenchus and other phytoparasitic nematodes.
低温可以确保重要生物标本的长期甚至无限期保存。线虫冷冻保存使得随时都能获得大量活线虫，特别是用于实验目的。最近收集到了新的伞滑刃线虫分离株，包括荒漠伞滑刃线虫（Rühm）古迪。该物种在意大利中北部的濒死橡树上以及从树皮甲虫细纹小蠹的 dauer 幼虫中被鉴定出来。因此，我们试图开发一种冷冻保存技术，用于长期保存所有可用的伞滑刃线虫物种。该技术包括一个快速冷却方案，即在液氮浴中浸泡，然后将冷冻样本储存在 -140℃的机械冰箱中。采用这种快速冷却方案处理的线虫存活率高于此前报道的使用缓慢冷却方法的存活率，并且适用于几种伞滑刃线虫和其他植物寄生线虫。
