Di Junwei, Zhang Min, Yao Kaian, Bi Shuping
Department of Chemistry, State Key Laboratory of Coordination Chemistry of China & Key Laboratory of MOE for Life Science, Nanjing University, Nanjing 210093, China.
Biosens Bioelectron. 2006 Aug 15;22(2):247-52. doi: 10.1016/j.bios.2006.01.001. Epub 2006 Feb 20.
The direct voltammetry of catalase (CAT) immobilized in silica sol-gel film in the presence of cysteine on gold electrode was investigated. The CAT electrode showed a pair of well-defined and quasi-reversible cyclic voltammetry peaks. It can be used as an electrochemical biosensor for the determination of hydrogen peroxide. The calibration range of H(2)O(2) was from 1 to 30 micromolL(-1) and the detection limit was 0.4 micromolL(-1) at a signal-to-noise ratio of 3. The interaction of CAT and aluminum ion was also investigated based on the CAT-modified electrode. The electrochemical activity of the CAT-modified electrode was increased with the addition of Al(3+). The experimental results of voltammetry and fluorescence spectroscopy indicated that the conformation of CAT molecule was altered by the formation of Al-CAT complex with Al(3+), which may influence the activity of CAT.
研究了在金电极上，半胱氨酸存在下，固定在硅溶胶 - 凝胶膜中的过氧化氢酶（CAT）的直接伏安法。CAT电极显示出一对定义明确且准可逆的循环伏安峰。它可用作测定过氧化氢的电化学生物传感器。H₂O₂的校准范围为1至30 μmolL⁻¹，在信噪比为3时检测限为0.4 μmolL⁻¹。还基于CAT修饰电极研究了CAT与铝离子的相互作用。随着Al³⁺的加入，CAT修饰电极的电化学活性增强。伏安法和荧光光谱法的实验结果表明，Al³⁺与CAT形成Al - CAT络合物改变了CAT分子的构象，这可能会影响CAT的活性。
Zotero 插件