Key Laboratory of Analytical Chemistry for Life Science, Ministry of Education of China, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
Talanta. 2009 Nov 15;80(1):224-30. doi: 10.1016/j.talanta.2009.06.056. Epub 2009 Jul 3.
A novel biosensor based on the silica-coated gold nanorods (GNRs@SiO(2)) and hydrophilic room temperature ionic liquid (RTIL) 1-butyl-3-methylimidazolium tetrafluroborate ([bmim][BF(4)]) was fabricated for the determination of hydrogen peroxide (H(2)O(2)) and nitrite. GNRs@SiO(2) can not only act as a binder to hinder [bmim][BF(4)] (RTIL) leaking from the electrode surface, but also provide a favorable microenvironment for direct electrochemistry of myoglobin (Mb). A pair of well-defined and quasi-reversible redox peaks of Mb was obtained at the GNRs@SiO(2)-Mb/RTIL-sol-gel composite film modified GCE (GNRs@SiO(2)-Mb/RTIL-sol-gel/GCE) through direct electron transfer between Mb and the underlying electrode. This biosensor showed an excellent electrocatalytic activity towards hydrogen peroxide and nitrite. The linear range for the determination of H(2)O(2) was from 0.2 to 180 microM with a detection limit of 0.12 microM based on the signal-to-noise ratio of 3. In addition, the biosensor also exhibited high selectivity, good reproducibility, and long-term stability. Therefore, this kind of composite film can provide an ideal matrix for protein immobilization and biosensor fabrication.
一种基于二氧化硅包覆金纳米棒（GNRs@SiO(2)）和亲水性室温离子液体（RTIL）1-丁基-3-甲基咪唑四氟硼酸盐([bmim][BF(4)])的新型生物传感器被制备用于测定过氧化氢（H(2)O(2)）和亚硝酸盐。GNRs@SiO(2)不仅可以作为一种粘合剂来阻止[bmim][BF(4)]（RTIL）从电极表面泄漏，而且还为肌红蛋白（Mb）的直接电化学提供了有利的微环境。通过 Mb 和底层电极之间的直接电子转移，在 GNRs@SiO(2)-Mb/RTIL-sol-gel 复合膜修饰的 GCE（GNRs@SiO(2)-Mb/RTIL-sol-gel/GCE）上获得了一对定义良好且准可逆的 Mb 氧化还原峰。该生物传感器对过氧化氢和亚硝酸盐表现出优异的电催化活性。测定 H(2)O(2)的线性范围为 0.2 至 180 microM，检测限为 0.12 microM，基于信噪比为 3。此外，该生物传感器还表现出高选择性、良好的重现性和长期稳定性。因此，这种复合膜可以为蛋白质固定化和生物传感器制造提供理想的基质。
