Wenk Markus, Droll Armin, Krähenbühl Stephan
Division of Clinical Pharmacology and Toxicology and Department of Research, University Hospital, 4031 Basel, Switzerland.
J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Mar 7;832(2):313-6. doi: 10.1016/j.jchromb.2006.01.015. Epub 2006 Feb 20.
In the present study, we developed a fast and reliable HPLC assay for the determination of the new triazole antifungal agent voriconazole in plasma, using a Chromolith RP 18e (100 mm x 4.6 mm) monolithic silica rod HPLC column. After liquid-liquid extraction, plasma samples were separated with a mobile phase consisting of ammoniumdihydrogencarbonate buffer (pH 5.8)-acetonitrile-tetrahydrofuran (72:25:3) at a flow-rate of 3.5 mL/min and UV detection at 255 nm. The retention times for voriconazole and internal standard (UK-115794) were 2.3 and 2.7 min, respectively, and total run time was 4 min. The calibration curves were linear between 0.05 and 10 microg/mL, and within-assay and between-assay coefficients of variation were <4%. The proposed assay for voriconazole in plasma is fast, sensitive and reliable, and, thus, well suited for routine therapeutic monitoring of patients and for pharmacokinetic studies. It can be predicted that the use of monolithic silica rod chromatography will substantially shorten the turn-around time in the therapeutic drug monitoring laboratory.
在本研究中，我们开发了一种快速可靠的高效液相色谱（HPLC）分析法，用于测定血浆中的新型三唑类抗真菌药物伏立康唑，采用的是Chromolith RP 18e（100 mm×4.6 mm）整体硅胶棒HPLC柱。液液萃取后，血浆样品用由碳酸氢铵缓冲液（pH 5.8）-乙腈-四氢呋喃（72:25:3）组成的流动相进行分离，流速为3.5 mL/min，并在255 nm处进行紫外检测。伏立康唑和内标（UK-115794）的保留时间分别为2.3分钟和2.7分钟，总运行时间为4分钟。校准曲线在0.05至10 μg/mL之间呈线性，批内和批间变异系数均<4%。所提出的血浆中伏立康唑的分析方法快速、灵敏且可靠，因此非常适合对患者进行常规治疗监测以及进行药代动力学研究。可以预计，整体硅胶棒色谱法的使用将大大缩短治疗药物监测实验室的周转时间。
