Department of Clinical Pharmacy, Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Mar 1;887-888:79-84. doi: 10.1016/j.jchromb.2012.01.015. Epub 2012 Jan 25.
A reversed phase ultra-performance liquid chromatography method was developed for the simultaneous quantitative determination of the systemically administered azoles voriconazole, posaconazole, isavuconazole, itraconazole and its metabolite hydroxy-itraconazole in plasma. The method involved a simple liquid-liquid extraction followed by ultra-performance liquid chromatography with an Acquity UPLC BEH Phenyl column and ultraviolet detection set at a wavelength of 260 nm. The assay was validated over the concentration range of 0.050-10,000 mg/l for all four azoles and one metabolite. The accuracies for the assay were 97-104%, inter- and intra-day coefficients of variation were <7.5% and extraction recoveries were 98% for voriconazole, posaconazole, isavuconazole and the internal standard (UK115794), and 79% and 88% for itraconazole and hydroxy-itraconazole respectively. This method proved to be simple, accurate, precise and fast and is currently in use in our laboratory for the quantitative analysis of these azoles for Therapeutic Drug Monitoring and pharmacokinetic research.
建立了一种反相超高效液相色谱法，用于同时定量测定系统给予的唑类药物伏立康唑、泊沙康唑、伊曲康唑及其代谢物羟基伊曲康唑在血浆中的浓度。该方法包括简单的液液萃取，然后进行超高效液相色谱分析，采用 Acquity UPLC BEH Phenyl 柱和紫外检测，检测波长为 260nm。该测定法在所有四种唑类药物和一种代谢物的浓度范围为 0.050-10,000mg/L 时具有良好的准确性。该测定法的准确度为 97-104%，日内和日间变异系数<7.5%，伏立康唑、泊沙康唑、伊曲康唑和内标（UK115794）的提取回收率为 98%，而伊曲康唑和羟基伊曲康唑的提取回收率分别为 79%和 88%。该方法简单、准确、精密、快速，目前在我们实验室中用于这些唑类药物的治疗药物监测和药代动力学研究的定量分析。
