Jakobsson P J, Odlander B, Steinhilber D, Rosén A, Claesson H E
Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden.
Biochem Biophys Res Commun. 1991 Jul 15;178(1):302-8. doi: 10.1016/0006-291x(91)91814-s.
Incubation of cell sonicates from monoclonal B cells with arachidonic acid led to the formation of leukotriene (LT) B4 and 5-hydroxy-eicosatetraenoic acid (5-HETE). In contrast, stimulation of intact B cells with the calcium ionophore A23187 +/- arachidonic acid did not, under similar conditions, lead to formation of LTB4. The identification of these products was based on reverse phase- and straight phase-HPLC analysis, UV-spectroscopy and gas chromatography-mass spectrometry. Cell sonicates of highly enriched human tonsillar B lymphocytes also converted arachidonic acid to LTB4 and 5-HETE. Activation of these cells with B cell mitogen and cytokines for three days led to an upregulation of 5-lipoxygenase activity. This study provides evidence for the biosynthesis of LTB4 from arachidonic acid in B cell lines and in normal human tonsillar B lymphocytes.
将单克隆B细胞的细胞超声裂解物与花生四烯酸一起孵育，会导致白三烯（LT）B4和5-羟基-二十碳四烯酸（5-HETE）的形成。相比之下，在类似条件下，用钙离子载体A23187 ± 花生四烯酸刺激完整的B细胞，并不会导致LTB4的形成。这些产物的鉴定基于反相和正相高效液相色谱分析、紫外光谱和气相色谱-质谱分析。高度富集的人扁桃体B淋巴细胞的细胞超声裂解物也能将花生四烯酸转化为LTB4和5-HETE。用B细胞有丝分裂原和细胞因子激活这些细胞三天会导致5-脂氧合酶活性上调。本研究为B细胞系和正常人扁桃体B淋巴细胞中由花生四烯酸生物合成LTB4提供了证据。
