Pfleger Kevin D G, Eidne Karin A
7TM Laboratory/Laboratory for Molecular Endocrinology, Western Australian Institute for Medical Research, UWA Centre for Medical Research, University of Western Australia, Nedlands, Perth, Western Australia 6009, Australia.
Nat Methods. 2006 Mar;3(3):165-74. doi: 10.1038/nmeth841.
Bioluminescence resonance energy transfer (BRET) is a straightforward biophysical technique for studying protein-protein interactions. It requires: (1) that proteins of interest and suitable controls be labeled with either a donor or acceptor molecule, (2) placement of these labeled proteins in the desired environment for assessing their potential interaction, and (3) use of suitable detection instrumentation to monitor resultant energy transfer. There are now several possible applications, combinations of donor and acceptor molecules, potential assay environments and detection system perturbations. Therefore, this review aims to demystify and clarify the important aspects of the BRET methodology that should be considered when using this technique.
生物发光共振能量转移（BRET）是一种用于研究蛋白质-蛋白质相互作用的直接生物物理技术。它需要：（1）将感兴趣的蛋白质和合适的对照用供体或受体分子进行标记；（2）将这些标记的蛋白质置于所需环境中以评估它们潜在的相互作用；（3）使用合适的检测仪器来监测产生的能量转移。目前有几种可能的应用、供体和受体分子的组合、潜在的检测环境以及检测系统干扰。因此，本综述旨在揭开BRET方法的神秘面纱，并阐明在使用该技术时应考虑的重要方面。
