生物发光共振能量转移：一种用于检测活细胞中蛋白质-蛋白质相互作用的新兴工具。

Bioluminescence resonance energy transfer: an emerging tool for the detection of protein-protein interaction in living cells.

作者信息

Gersting Søren W, Lotz-Havla Amelie S, Muntau Ania C

机构信息

Department of Molecular Pediatrics, Dr. von Hauner Children's Hospital, Ludwig-Maximilians-University, Münich 80337, Germany.

出版信息

Methods Mol Biol. 2012;815:253-63. doi: 10.1007/978-1-61779-424-7_19.

DOI:10.1007/978-1-61779-424-7_19

PMID:22130997

Abstract

In the field of proteomics, numerous advanced technologies have evolved that aim to provide the molecular data necessary for an in-depth understanding of biological processes. Protein-protein interactions (PPI) are at the heart of cellular function and a milestone yet to be achieved is the mapping of a complete human interactome. Bioluminescence resonance energy transfer (BRET) has become a popular technique to investigate PPI. As BRET enables the detection of PPI in living cells, problems associated with in vitro biochemical assays can be circumvented, thus making BRET a powerful tool for monitoring interactions of virtually all kinds of protein species.

摘要

在蛋白质组学领域，已经涌现出众多先进技术，旨在提供深入理解生物过程所需的分子数据。蛋白质-蛋白质相互作用（PPI）是细胞功能的核心，而绘制完整的人类相互作用组图谱仍是一个有待实现的里程碑。生物发光共振能量转移（BRET）已成为研究PPI的一种流行技术。由于BRET能够在活细胞中检测PPI，因此可以规避与体外生化分析相关的问题，从而使BRET成为监测几乎所有种类蛋白质相互作用的强大工具。