Zhong Youmin, Weininger Marilyn, Pirbhai Mustak, Dong Feng, Zhong Guangming
Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA.
J Infect. 2006 Dec;53(6):408-14. doi: 10.1016/j.jinf.2005.12.028. Epub 2006 Feb 20.
It has been reported that all major chlamydial species can inhibit host cell apoptosis and the Chlamydia trachomatis antiapoptotic activity is correlated with inhibition of activation of the proapoptotic multidomain Bcl-2 proteins Bax and Bak and degradation of BH3-only domain Bcl-2 proteins such as Puma. The current study is to test whether the more invasive species can also suppress host apoptosis through inhibition of Bax and Bak activation. We compared the effects of the three invasive chlamydial species C. muridarum, C. caviae, C. psittaci with that of C. trachomatis on host cell Bax and Bak activation. We found that these chlamydial species not only failed to activate Bax and Bak but also significantly inhibited Bax and Bak activation, mitochondrial cytochrome c release and caspase 3 activation induced by staurosporine. These results have demonstrated that inhibition of host cell apoptosis pathways mediated by Bax and Bak activation is a common property of the major chlamydial species.
据报道，所有主要衣原体物种均可抑制宿主细胞凋亡，沙眼衣原体的抗凋亡活性与抑制促凋亡多结构域Bcl-2蛋白Bax和Bak的激活以及仅含BH3结构域的Bcl-2蛋白（如Puma）的降解相关。当前研究旨在测试更具侵袭性的物种是否也能通过抑制Bax和Bak激活来抑制宿主细胞凋亡。我们比较了三种侵袭性衣原体物种——鼠衣原体、豚鼠衣原体、鹦鹉热衣原体与沙眼衣原体对宿主细胞Bax和Bak激活的影响。我们发现，这些衣原体物种不仅未能激活Bax和Bak，还显著抑制了由星形孢菌素诱导的Bax和Bak激活、线粒体细胞色素c释放以及半胱天冬酶3激活。这些结果表明，抑制由Bax和Bak激活介导的宿主细胞凋亡途径是主要衣原体物种的共同特性。
