用于热带利什曼原虫鉴别诊断的直接种特异性PCR检测方法的开发。
Development of a direct species-specific PCR assay for differential diagnosis of Leishmania tropica.
作者信息
Jirkù Milan, Zemanová Eva, Al-Jawabreh Amer, Schönian Gabriele, Lukes Julius
机构信息
Institute of Parasitology, Czech Academy of Sciences, 37005 Ceské Budejovice, Czech Republic.
出版信息
Diagn Microbiol Infect Dis. 2006 May;55(1):75-9. doi: 10.1016/j.diagmicrobio.2005.12.001. Epub 2006 Feb 20.
Abstract
We have developed a polymerase chain reaction assay for differential diagnosis of Leishmania tropica, based on simple amplification of the target region. The assay detects less than 5 protozoan cells, was tested on human samples and an experimentally infected animal, and is appropriate for clinical laboratories in countries where leishmaniasis is endemic.
摘要
我们基于目标区域的简单扩增，开发了一种用于热带利什曼原虫鉴别诊断的聚合酶链反应检测方法。该检测方法可检测出少于5个原生动物细胞，已在人类样本和实验感染动物身上进行了测试，适用于利什曼病流行国家的临床实验室。
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