Langer S, Botteck N M, Bosse B, Reimer K, Vogt P M, Steinau H-U, Mueller S
Department of Plastic and Hand Surgery, Burn Center, University Hospital Bergmannsheil, Ruhr University Bochum, Bochum, Germany.
Eur Surg Res. 2006;38(1):27-34. doi: 10.1159/000091524. Epub 2006 Feb 14.
Polyvinylpyrrolidone-iodine liposomal hydrogel (PVP-ILH) is a hydrogel formulation based on polyvinylpyrrolidone-iodine (PVP-I) and liposomes. The beneficial effects of PVP-ILH on wound healing have been previously shown. The aim of this study was to investigate the effects of topically applied PVP-ILH on wound microcirculation.
Experiments were performed on wounds in male SKH1-hr hairless mice (n = 48). Mice were randomized into five treatment groups: mice treated with polyacrylic acid (PAA) and PAA 1:10 as well as PVP-ILH and PVP-ILH 1:10. Mice treated with sodium chloride served as control. Immediately as well as 3, 7, and 14 days after wounding, intravital fluorescent microscopy (IFM) was performed to determine wound surface area and standard microcirculatory parameters.
Topically administered PVP-ILH reduced wound size significantly faster compared to controls. Standard microcirculatory parameters, e.g. functional capillary density (FCD) and plasma leakage, showed no differences. FCD increases in all groups after wound creation. Using PVP-ILH, a trend towards higher FCD was observed.
The wound model in hairless mice in combination with IFM is suitable to qualitatively assess wound microcirculation over a period of 2 weeks even after topical application of pigmented ointments. PVP-ILH showed a positive effect on dermal wound healing and wound microcirculation.
聚乙烯吡咯烷酮碘脂质体水凝胶（PVP - ILH）是一种基于聚乙烯吡咯烷酮碘（PVP - I）和脂质体的水凝胶制剂。先前已表明PVP - ILH对伤口愈合具有有益作用。本研究的目的是探讨局部应用PVP - ILH对伤口微循环的影响。
对雄性SKH1 - hr无毛小鼠（n = 48）的伤口进行实验。小鼠被随机分为五个治疗组：用聚丙烯酸（PAA）和PAA 1:10以及PVP - ILH和PVP - ILH 1:10治疗的小鼠。用氯化钠治疗的小鼠作为对照。在受伤后即刻以及3、7和14天，进行活体荧光显微镜检查（IFM）以确定伤口表面积和标准微循环参数。
与对照组相比，局部应用PVP - ILH能显著更快地减小伤口大小。标准微循环参数，如功能性毛细血管密度（FCD）和血浆渗漏，无差异。伤口形成后所有组的FCD均增加。使用PVP - ILH时，观察到FCD有升高趋势。
无毛小鼠的伤口模型与IFM相结合，即使在局部应用色素软膏后，也适用于在2周内定性评估伤口微循环。PVP - ILH对皮肤伤口愈合和伤口微循环显示出积极作用。
