Strüder Daniel, Grambow Eberhard, Klar Ernst, Mlynski Robert, Vollmar Brigitte
Department of Otorhinolaryngology, Head and Neck Surgery "Otto Koerner", Rostock University Medical Center;
Department of General, Thoracic, Vascular and Transplantation Surgery, Rostock University Medical Center.
J Vis Exp. 2017 Apr 2(122):55174. doi: 10.3791/55174.
Thrombotic complications of vascular diseases are one leading cause of morbidity and mortality in industrial nations. Due to the complex interactions between cellular and non-cellular blood components during thrombus formation, reliable studies of the physiology and pathophysiology of thrombosis can only be performed in vivo. Therefore, this article presents an ear model in hairless mice and focuses on the in vivo analysis of microcirculation, thrombus formation, and thrombus evolution. By using intravital fluorescence microscopy and the intravenous (iv) application of the respective fluorescent dyes, a repetitive analysis of microcirculation in the auricle can easily be performed, without the need for surgical preparation. Furthermore, this model can be adapted for in vivo studies of different issues, including wound healing, reperfusion injury, or angiogenesis. In summary, the ear of hairless mice is an ideal model for the in vivo study of skin microcirculation in physiological or pathophysiological conditions and for the evaluation of its reaction to different systemic or topical treatments.
血管疾病的血栓并发症是工业化国家发病和死亡的主要原因之一。由于血栓形成过程中细胞和非细胞血液成分之间存在复杂的相互作用，因此只有在体内才能对血栓形成的生理学和病理生理学进行可靠的研究。因此，本文介绍了一种无毛小鼠耳部模型，并着重于对微循环、血栓形成和血栓演变进行体内分析。通过使用活体荧光显微镜以及静脉注射（iv）相应的荧光染料，可以轻松地对耳廓中的微循环进行重复分析，而无需进行手术准备。此外，该模型可适用于不同问题的体内研究，包括伤口愈合、再灌注损伤或血管生成。总之，无毛小鼠的耳朵是在生理或病理生理条件下对皮肤微循环进行体内研究以及评估其对不同全身或局部治疗反应的理想模型。
