Thomas M J, Smith S, Pang J A
Department of Biochemistry, Wake Forest University Medical Center, Bowman Gray School of Medicine, Winston-Salem, North Carolina 27103.
Free Radic Res Commun. 1991;12-13 Pt 1:53-7. doi: 10.3109/10715769109145767.
The polymorphonuclear leukocyte secretes both O2- and H2O2 when stimulated by various soluble or particulate stimuli. Since a reaction involving iron, O2-, and H2O2 could generate the hydroxyl radical (HO.) there has been speculation that the HO. may participate in the bactericidal activity of the neutrophil. A variety of water-soluble HO. scavengers have been used to test for the participation of HO. and the results imply that HO. might participate. However, other workers have not been able to detect the formation of significant amounts of HO. by the activated neutrophil. We have examined the effect of several commonly used HO. radical scavengers on the ability of the neutrophil to secrete O2- and H2O2. Several of these compounds actively inhibit secretion without affecting the viability of the neutrophil. After considering the various complications inherent in using water soluble radical scavengers, we suggest that they only be used with well defined experimental systems.
多形核白细胞在受到各种可溶性或颗粒性刺激时会分泌超氧阴离子（O2-）和过氧化氢（H2O2）。由于涉及铁、超氧阴离子和过氧化氢的反应可生成羟基自由基（HO·），因此有人推测羟基自由基可能参与中性粒细胞的杀菌活性。已使用多种水溶性羟基自由基清除剂来测试羟基自由基的参与情况，结果表明羟基自由基可能参与其中。然而，其他研究人员未能检测到活化的中性粒细胞形成大量的羟基自由基。我们研究了几种常用的羟基自由基清除剂对中性粒细胞分泌超氧阴离子和过氧化氢能力的影响。其中几种化合物可有效抑制分泌，而不影响中性粒细胞的活力。在考虑了使用水溶性自由基清除剂所固有的各种复杂情况后，我们建议仅在定义明确的实验系统中使用它们。
