Herrmann K, Heckmann M, Kulozik M, Haustein U F, Krieg T
Department of Dermatology, Universität Leipzig, München, Germany.
J Invest Dermatol. 1991 Aug;97(2):219-22. doi: 10.1111/1523-1747.ep12480157.
Total RNA was extracted from skin biopsies of nine patients suffering from systemic sclerosis (SSc). Steady-state mRNA levels of collagen alpha 1(I) and alpha 1(III), collagenase, fibronectin, and beta-actin were studied using specific cDNA probes and compared to those of 12 sex- and age-matched healthy individuals. There was a more than three-fold elevation of collagen I mRNA levels in SSc skin compared to controls. No difference was found, however, for collagen III, collagenase, and fibronectin mRNA levels in SSc and control biopsies. The selective increase of collagen alpha 1(I) mRNA levels indicates a specific alteration of fibroblast metabolism in scleroderma. Analysis of mRNA levels in skin biopsies might not only offer a direct approach to the understanding of the pathophysiology of SSc, but also facilitate the monitoring of fibrotic activity in SSc patients during therapeutic trials.
从9名系统性硬化症（SSc）患者的皮肤活检样本中提取总RNA。使用特异性cDNA探针研究了胶原蛋白α1（I）和α1（III）、胶原酶、纤连蛋白和β-肌动蛋白的稳态mRNA水平，并与12名年龄和性别匹配的健康个体进行比较。与对照组相比，SSc皮肤中胶原蛋白I mRNA水平升高了三倍多。然而，在SSc和对照活检样本中，胶原蛋白III、胶原酶和纤连蛋白的mRNA水平没有差异。胶原蛋白α1（I）mRNA水平的选择性增加表明硬皮病中成纤维细胞代谢发生了特异性改变。分析皮肤活检样本中的mRNA水平不仅可能为理解SSc的病理生理学提供直接方法，还有助于在治疗试验期间监测SSc患者的纤维化活动。
