Kadota J, Oda H, Sakito O, Sawa H, Morikawa N, Mukae H, Senju R, Tomono K, Fukushima K, Hiratani K
Second Department of Internal Medicine, Nagasaki University School of Medicine.
Kansenshogaku Zasshi. 1991 Mar;65(3):277-85. doi: 10.11150/kansenshogakuzasshi1970.65.277.
The "low dose and long term" erythromycin (EM) therapy has been reported as effective (or useful) in chronic respiratory tract disease with pseudomonas (P.) infection including diffuse panbronchiolitis (DPB), however the mode of action is still obscure. Therefore in this study we have examined the effect of EM on the interaction between P. aeruginosa and human polymorphonuclear leukocyte (PMN) in vitro. The efficiency of intracellular killing and the ability of superoxide production were employed to evaluate the PMN functions. For the first step, the following results were obtained; 1) Pretreatment of PMN with 20 micrograms/ml EM did not affect the killing ability of PMNs against opsonized P. aeruginosa of standard and clinical isolate from DPB patient. 2) Superoxide production from PMNs was observed by phagocytosis of P. aeruginosa in the presence of serum. This was not affected by exposure of PMNs to 20 micrograms/ml EM even with increased ratios of bacteria to cells. 3) Pretreatment of P. aeruginosa with 20 micrograms/ml EM before opsonization enhanced the killing ability of PMNs in both standard and clinical isolate. 4) Pretreatment of P. aeruginosa with 20 micrograms/ml EM resulted in no effect on superoxide production from PMNs by phagocytosis of the bacteria. These results indicate that EM may modify a certain step of the interaction between bacteria and intracellular host defence mechanisms. Therefore for the second step, we have investigated the susceptibility of EM-exposed bacteria to killing by the cell free (glucose oxidase-glucose) system, which will detect the enzymatic generation of hydrogen peroxide (H2O2). The following results were observed; 1) EM-exposed bacteria was more susceptible to killing than control bacteria.(ABSTRACT TRUNCATED AT 250 WORDS)
据报道，“低剂量长期”红霉素（EM）疗法对包括弥漫性泛细支气管炎（DPB）在内的伴有铜绿假单胞菌（P.）感染的慢性呼吸道疾病有效（或有用），但其作用方式仍不清楚。因此，在本研究中，我们检测了EM对铜绿假单胞菌与人多形核白细胞（PMN）体外相互作用的影响。采用细胞内杀伤效率和超氧化物产生能力来评估PMN的功能。第一步，获得了以下结果：1）用20微克/毫升EM预处理PMN，不影响PMN对标准菌株和DPB患者临床分离株的调理铜绿假单胞菌的杀伤能力。2）在血清存在下，通过吞噬铜绿假单胞菌观察到PMN产生超氧化物。即使细菌与细胞的比例增加，将PMN暴露于20微克/毫升EM也不会影响超氧化物的产生。3）在调理前用20微克/毫升EM预处理铜绿假单胞菌，可增强标准菌株和临床分离株中PMN的杀伤能力。4）用20微克/毫升EM预处理铜绿假单胞菌，对通过吞噬细菌产生的PMN超氧化物没有影响。这些结果表明，EM可能会改变细菌与细胞内宿主防御机制相互作用的某个步骤。因此，在第二步中，我们研究了经EM处理的细菌对无细胞（葡萄糖氧化酶-葡萄糖）系统杀伤的敏感性，该系统将检测过氧化氢（H2O2）的酶促生成。观察到以下结果：1）经EM处理的细菌比对照细菌更易被杀伤。（摘要截断于250字）
