Baldus S E, Mönig S P, Zirbes T K, Thakran J, Köthe D, Köppel M, Hanisch F-G, Thiele J, Schneider P M, Hölscher A H, Dienes H P
Intitute of Pathology, University of Düesseldorf, Düesseldorf, Germany.
Histol Histopathol. 2006 May;21(5):503-10. doi: 10.14670/HH-21.503.
Lewis(y) (Le(y)), also designated CD174, represents a carbohydrate blood group antigen which is strongly expressed in neoplastic gastrointestinal tissues. Previous reports indicated an association between Le(y) expression and apoptosis. Therefore, we tried to elucidate its clinicopathological relevance in a series of 160 gastric and 215 colorectal carcinomas by immunohistochemical detection of Le(y) and visualization of apoptotic cells applying the in-situ-end labelling (ISEL) method, followed by semiquantitative scoring of the specimens. In both gastric as well as colorectal carcinomas, between 40 and 50% of the cases were Le(y) reactive. Signet-ring cell carcinomas of the stomach exhibited a significantly stronger Le(y) expression compared to other tumor types. In colorectal cancers, Le(y) was associated with increased tumor staging, showing the strongest positivity in stage IV. Further correlations with clinicopathological variables or prognosis were not observed. On the other hand, the amount of apoptotic cells was significantly reduced in mucinous adenocarcinomas of the colorectum compared to non-mucinous carcinomas. Scoring of apoptotic cells did not result in any other clinicopathologically relevant correlations. In addition, a significant association between Le(y) antigen expression and apoptosis score could not be established. Therefore, the hypothesis of a functional relationship between these two aspects of gastrointestinal tumor biology is not confirmed by our data.
Lewis(y)（Le(y)），也被称为CD174，是一种碳水化合物血型抗原，在胃肠道肿瘤组织中高表达。先前的报道表明Le(y)表达与细胞凋亡之间存在关联。因此，我们通过免疫组化检测Le(y)并应用原位末端标记（ISEL）方法对凋亡细胞进行可视化，随后对标本进行半定量评分，试图阐明其在160例胃癌和215例结直肠癌中的临床病理相关性。在胃癌和结直肠癌中，40%至50%的病例对Le(y)呈反应性。胃印戒细胞癌与其他肿瘤类型相比，Le(y)表达明显更强。在结直肠癌中，Le(y)与肿瘤分期增加相关，在IV期显示出最强的阳性。未观察到与其他临床病理变量或预后的进一步相关性。另一方面，与非黏液腺癌相比，结直肠黏液腺癌中的凋亡细胞数量显著减少。对凋亡细胞的评分未得出任何其他与临床病理相关的相关性。此外，无法确定Le(y)抗原表达与凋亡评分之间存在显著关联。因此，我们的数据未证实胃肠道肿瘤生物学这两个方面之间存在功能关系的假设。
