Harty R N, Yalamanchili R R, O'Callaghan D J
Department of Microbiology and Immunology, Louisiana State University Medical Center, Shreveport 71130-3932.
Virology. 1991 Aug;183(2):830-3. doi: 10.1016/0042-6822(91)91020-h.
Defective interfering particles (DIPs) of equine herpesvirus type 1 (EHV-1) are biologically active, in that they mediate the coestablishment of oncogenic transformation and persistent infection in permissive, primary hamster embryo fibroblasts. The DIP genome is composed of EHV-1 sequences originating from the L-terminus (mapping units (m.u.) 0.00-0.023), the junction of the unique long (UL) region and the internal inverted repeat (IR) (m.u. 0.78-0.79 and 0.99-1.00), and the central portion of the IR (m. u. 0.83-0.87 and 0.91-0.95). The nature of one of the genes (UL1) mapping at the L-terminus was analyzed at the RNA level by Northern blot hybridization and S1 nuclease analyses. These data, and DNA sequencing analyses reported previously revealed that the UL1 gene: (1) contains a major open reading frame (ORF) of 258 amino acids, (2) is a homologue of the ORF2 gene of varicella zoster virus (VZV), (3) is conserved in the genome of DIPs of EHV-1, (4) encodes a 1.2-kb early (E) mRNA that is transcribed toward the short region of the genome, (5) utilizes a transcription initiation site approximately 1,120 nucleotides from the L-terminus, and (6) utilizes a transcription termination site approximately 2211 nucleotides from the L-terminus. These initial studies serve as the basis of future work to determine the function of the UL1 gene in cytolytic infection, and its potential role in EHV-1 persistent infection.
1型马疱疹病毒（EHV-1）的缺陷干扰颗粒（DIPs）具有生物活性，因为它们在允许性原代仓鼠胚胎成纤维细胞中介导致癌转化和持续感染的共同建立。DIP基因组由源自L末端（图谱单位（m.u.）0.00 - 0.023）、独特长（UL）区域与内部反向重复序列（IR）的交界处（m.u. 0.78 - 0.79和0.99 - 1.00）以及IR的中央部分（m.u. 0.83 - 0.87和0.91 - 0.95）的EHV-1序列组成。通过Northern印迹杂交和S1核酸酶分析在RNA水平分析了位于L末端的一个基因（UL1）的性质。这些数据以及先前报道的DNA测序分析表明，UL1基因：（1）包含一个258个氨基酸的主要开放阅读框（ORF），（2）是水痘带状疱疹病毒（VZV）的ORF2基因的同源物，（3）在EHV-1的DIP基因组中保守，（4）编码一个1.2 kb的早期（E）mRNA，该mRNA朝着基因组的短区域转录，（5）利用一个距L末端约1120个核苷酸的转录起始位点，（6）利用一个距L末端约2211个核苷酸的转录终止位点。这些初步研究为未来确定UL1基因在溶细胞感染中的功能及其在EHV-1持续感染中的潜在作用的工作奠定了基础。
