炭疽芽孢杆菌中的常规无标记基因替换
Routine markerless gene replacement in Bacillus anthracis.
作者信息
Janes Brian K, Stibitz Scott
机构信息
Division of Bacterial Products, CBER/FDA, 8800 Rockville Pike, Bethesda, MD 20892, USA.
出版信息
Infect Immun. 2006 Mar;74(3):1949-53. doi: 10.1128/IAI.74.3.1949-1953.2006.
Abstract
An improved genetic tool suitable for routine markerless allelic exchange in Bacillus anthracis has been constructed. Its utility was demonstrated by the introduction of insertions, deletions, and missense mutations on the chromosome and plasmid pXO1 of the Sterne strain of B. anthracis.
摘要
构建了一种改进的遗传工具，适用于炭疽芽孢杆菌中常规的无标记等位基因交换。通过在炭疽芽孢杆菌斯特恩菌株的染色体和质粒pXO1上引入插入、缺失和错义突变，证明了其效用。
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