Stepanov A S, Gavrilov S V, Puzanova O B, Grigor'eva T M, Azizbekian R R
Mol Gen Mikrobiol Virusol. 1989 Jan(1):14-9.
Possibility of plasmid transduction in Bacillus anthracis vaccine strains Sterne and STI-1 by bacteriophage CP54ant having an increased ability of adsorbtion and a shortened period of latent development in Bacillus anthracis cells has been isolated. The main parameters of plasmid transduction by the bacteriophage have been established for the plasmid pTG141 (TcR). They include the effect of multiplicity of infection, the level of UV-inactivation of bacteriophage, the presence of antiphage serum in the incubation medium. Plasmid transduction by the mutant phage CP54ant was found to be more efficient as compared with the one by the parent phage. The isolated transductants served as donors of the transduced plasmid for Bacillus anthracis and Bacillus thuringiensis strains.
已分离出噬菌体CP54ant，其在炭疽芽孢杆菌细胞中具有增强的吸附能力和缩短的潜伏发育周期，可使炭疽芽孢杆菌疫苗株Sterne和STI-1发生质粒转导。已确定噬菌体对质粒pTG141（TcR）进行质粒转导的主要参数。这些参数包括感染复数的影响、噬菌体紫外线灭活水平、孵育培养基中抗噬菌体血清的存在。发现突变噬菌体CP54ant进行的质粒转导比亲本噬菌体更有效。分离出的转导子可作为炭疽芽孢杆菌和苏云金芽孢杆菌菌株转导质粒的供体。
