Kwon Chang-Hyuk, Zhou Jing, Li Yanjiao, Kim Ki Woo, Hensley Lori L, Baker Suzanne J, Parada Luis F
Center for Developmental Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9133, USA.
Genesis. 2006 Mar;44(3):130-5. doi: 10.1002/gene.20197.
To establish genetic tools for conditional gene deletion in mouse neurons, we generated two independent neuron-specific enolase (Nse)-cre transgenic lines. The transgenic line termed Nse-cre(CK1) showed cre activity in most neuronal regions in the nervous system, while the Nse-cre(CK2) line exhibited a unique cre activity that has not been reported in other cre transgenic lines. Nse-cre(CK2) cre activity was detectable from embryogenesis and mostly restricted to neuronal regions. In postnatal brain, the Nse-cre(CK2) line exhibited cre activity limited to differentiated neurons in the cerebral cortex and hippocampus. Cre activity was assayed in several internal organs and sporadic activity was limited to the kidney and testis. We conclude that these cre lines will be useful for studying loss of gene function in specific neuronal populations.
为了建立用于小鼠神经元条件性基因缺失的遗传工具，我们构建了两个独立的神经元特异性烯醇化酶（Nse）-cre转基因品系。名为Nse-cre(CK1)的转基因品系在神经系统的大多数神经元区域显示出cre活性，而Nse-cre(CK2)品系表现出一种独特的cre活性，这在其他cre转基因品系中尚未见报道。Nse-cre(CK2)的cre活性在胚胎发育过程中即可检测到，且大多局限于神经元区域。在出生后的大脑中，Nse-cre(CK2)品系的cre活性仅限于大脑皮层和海马体中已分化的神经元。在几个内脏器官中检测cre活性，发现零星活性仅限于肾脏和睾丸。我们得出结论，这些cre品系将有助于研究特定神经元群体中的基因功能丧失。
