Sato Tomomi, Takahoko Mikako, Okamoto Hitoshi
Laboratory for Developmental Gene Regulation, Brain Science Institute, RIKEN, Japan.
Genesis. 2006 Mar;44(3):136-42. doi: 10.1002/gene.20196.
A simple and efficient procedure for labeling neurons is a prerequisite for investigating the development of neural networks in zebrafish. To label neurons we used Kaede, a fluorescent protein with a photoconversion property allowing conversion from green to red fluorescence following irradiation with UV or violet light. We established a zebrafish stable transgenic line, Tg(HuC:Kaede), expressing Kaede in neurons under the control of the HuC promoter. This transgenic line was used to label a small number of neurons in the trigeminal ganglion. Also, using embryos injected with the transgene, we labeled peripheral axon arbors of a Rohon-Beard neuron at 4 days postfertilization and observed the dendrite development of a tectal neuron for 3 days. These data indicate that Kaede is a useful tool to selectively label neural networks in zebrafish.
一种简单有效的神经元标记方法是研究斑马鱼神经网络发育的先决条件。为了标记神经元，我们使用了Kaede，一种具有光转换特性的荧光蛋白，在用紫外线或紫光照射后可从绿色荧光转换为红色荧光。我们建立了一个斑马鱼稳定转基因品系Tg(HuC:Kaede)，在HuC启动子的控制下在神经元中表达Kaede。该转基因品系用于标记三叉神经节中的少量神经元。此外，我们使用注射了转基因的胚胎，在受精后4天标记了罗霍恩-比尔兹神经元的外周轴突分支，并观察了顶盖神经元的树突发育3天。这些数据表明，Kaede是选择性标记斑马鱼神经网络的有用工具。
