[杜鹃兰块茎的化学成分]

[Chemical constituents from tuber of Cremastra appendiculata].

作者信息

Xia Wen-bin, Xue Zhen, Li Shuai, Wang Su-juan, Yang Yong-chun, He Ding-xiang, Ran Guang-lun, Kong Ling-zhong, Shi Jian-gong

机构信息

Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing.

出版信息

Zhongguo Zhong Yao Za Zhi. 2005 Dec;30(23):1827-30.

PMID:16499019

Abstract

OBJECTIVE

To investigate the chemical constituents from the tuber of the planted Cremastra appendiculata.

METHOD

The compounds were isolated by column chromatography over silica gel, Sephadex LH-20 and RP-HPLC, and their structures were elucidated on the basis of spectroscopic analysis.

RESULT

Eight compounds were isolated, and identified as cirrhopetalanthrin (I), 7-hydroxy-4-methoxyphenanthrene-2-O-beta-D-glucoside (II), 4-(2-hydroxyethyl)-2-methoxyphenyl-1-O-beta-D-glucopyranoside (III), tyrosol 8-O-beta-D-gluco-pyranoside (IV), vanilloloside (V), p-hydroxybenzaldehyde (VI), sucrose (VII), adenosine (VIII).

CONCLUSION

These compounds are isolated from this plant for the first time. All compounds were evaluated against human colon cancer (HCT-8), human hepatoma (Bel7402), human stomach cancer(BGC-823), human lung adenocarcinoma (A549), human breast cancer (MCF-7), and human ovarian cancer (A2780) cell lines, and cirrhopetalanthrin (I) showed non-selective moderate cytotoxicity with IC50 values of 8.4-13.3 micromol x L(-1), and other compounds were inactive.

摘要

目的

研究人工种植的杜鹃兰块茎中的化学成分。

方法

通过硅胶柱色谱、葡聚糖凝胶LH - 20柱色谱和反相高效液相色谱分离化合物，并根据光谱分析确定其结构。

结果

分离得到8个化合物，分别鉴定为卷瓣兰素（I）、7 - 羟基 - 4 - 甲氧基菲 - 2 - O - β - D - 葡萄糖苷（II）、4 - （2 - 羟乙基） - 2 - 甲氧基苯基 - 1 - O - β - D - 吡喃葡萄糖苷（III）、酪醇8 - O - β - D - 葡萄糖苷（IV）、香草醇苷（V）、对羟基苯甲醛（VI）、蔗糖（VII）、腺苷（VIII）。

结论

这些化合物均为首次从该植物中分离得到。对所有化合物进行了针对人结肠癌（HCT - 8）、人肝癌（Bel7402）、人胃癌（BGC - 823）、人肺腺癌（A549）、人乳腺癌（MCF - 7）和人卵巢癌（A2780）细胞系的活性评价，卷瓣兰素（I）表现出非选择性的中等细胞毒性，IC50值为8.4 - 13.3 μmol·L⁻¹，其他化合物无活性。