Murdin A D, Kameda A, Murray M G, Wimmer E
Department of Microbiology, State University of New York, Stony Brook 11794.
Microb Pathog. 1991 Jan;10(1):39-45. doi: 10.1016/0882-4010(91)90064-h.
Three poliovirus hybrids, modified in neutralization antigenic sites (NAgs) I or II, were characterized for several phenotypic traits. The modifications to the capsid interfered with some stage of the life-cycle of the virus, since all three hybrids were growth-impaired in comparison to poliovirus type 1 (Mahoney) [PV1 (M)], the wild-type parent virus. All hybrids exhibited a reduced growth rate and a small-plaque phenotype, but they were not temperature sensitive. Furthermore, only one hybrid was slightly less stable to heating than the parent virus; the other two were as stable as the parent. Therefore, decreased thermal stability of the capsid is not an important cause of the poor growth characteristics of these hybrids.
三种在中和抗原位点（NAgs）I或II中进行了修饰的脊髓灰质炎病毒杂交株，对其若干表型特征进行了表征。衣壳的修饰干扰了病毒生命周期的某个阶段，因为与野生型亲本病毒1型脊髓灰质炎病毒（Mahoney）[PV1（M）]相比，所有三种杂交株的生长均受到损害。所有杂交株均表现出生长速率降低和小蚀斑表型，但它们对温度不敏感。此外，只有一种杂交株在加热时的稳定性略低于亲本病毒；另外两种与亲本一样稳定。因此，衣壳热稳定性降低不是这些杂交株生长特性不佳的重要原因。
