Kharfi Abdelaziz, Bureau Marc, Giguère Yves, Moutquin Jean-Marie, Forest Jean-Claude
Département d'Obstétrique-Gynécologie, Centre Universitaire de Sherbrooke, Québec, Canada.
Clin Biochem. 2006 Jun;39(6):646-51. doi: 10.1016/j.clinbiochem.2006.01.016. Epub 2006 Feb 23.
To analyse in situ the placental expression of tumor necrosis factor-alpha (TNF-alpha), its receptors TNFRp55 and TNFRp75, and apoptosis in normotensive and preeclamptic pregnancies.
We simultaneously analyzed the immunostaining of TNF-alpha, its receptors and apoptosis in term placentas of 15 patients with preeclampsia and 15 normotensive pregnant women as controls.
In normotensive villi TNF-alpha and TNFRp75 were expressed more in syncytiotrophoblasts than cytotrophoblasts or stromal cells, and were almost absent in endothelial cells. TNFRp55 was expressed uniformly in all types of placental cells. Apoptosis was more marked in syncytiotrophoblasts than cytotrophoblasts. In preeclamptic trophoblasts apoptosis was exaggerated whereas expression of TNF-alpha and its receptors remained unchanged.
Placental expression of TNF-alpha and TNFRp75 appear inter-adaptative and follow the same pattern, whereas TNFRp55 and TNF-alpha appear independent. In addition, the exaggerated apoptosis of preeclamptic trophoblasts may be dependent on factors other than the TNF-alpha system alone.
原位分析肿瘤坏死因子-α（TNF-α）及其受体TNFRp55和TNFRp75在正常血压和子痫前期妊娠胎盘组织中的表达情况以及细胞凋亡情况。
我们同时分析了15例子痫前期患者和15例正常血压孕妇作为对照的足月胎盘组织中TNF-α、其受体以及细胞凋亡的免疫染色情况。
在正常血压的绒毛组织中，TNF-α和TNFRp75在合体滋养层细胞中的表达高于细胞滋养层细胞或基质细胞，在内皮细胞中几乎不表达。TNFRp55在所有类型的胎盘细胞中均呈均匀表达。合体滋养层细胞中的细胞凋亡比细胞滋养层细胞更为明显。在子痫前期的滋养层细胞中，细胞凋亡加剧，而TNF-α及其受体的表达保持不变。
TNF-α和TNFRp75在胎盘组织中的表达似乎相互适应并遵循相同模式，而TNFRp55和TNF-α的表达似乎相互独立。此外，子痫前期滋养层细胞中过度的细胞凋亡可能不仅仅依赖于TNF-α系统这一单一因素。
