Basu Jayasri, Agamasu Enyonam, Bendek Bolek, Salafia Carolyn M, Mishra Aruna, Benfield Nerys, Prasad Priya, Mikhail Magdy
a Department of Obstetrics & Gynecology , Bronx Lebanon Hospital Center , Bronx , NY , USA.
J Matern Fetal Neonatal Med. 2016 Dec;29(24):3934-8. doi: 10.3109/14767058.2016.1156668. Epub 2016 Mar 18.
Placental tumor necrosis factor-α (TNF-α) is a cell signaling protein. During pregnancy, TNF-α induces synthesis of matrix metalloproteinases (MMPs) which allows cytotrophoblasts to reach the spiral arteries deeper within the uterine decidua. TNF-α also augments apoptosis of vascular smooth muscle cells surrounding these arteries. In this study, chorionic villi TNF-α protein expression throughout normal human gestation were investigated.
Placental chorionic villi tissues obtained from elective surgical terminations of pregnancy and from uncomplicated term births were assayed using EIA kits (Cayman Chemicals, Ann Arbor, MI, Item # 589201).
The median, 25th percentile and 75th percentile values in the first (N = 99), second (N = 58) and third trimester (N = 42) were: 36.46, 27.25, 45.90 pg/100 mg tissue; 55.43, 40.09, 110.88 pg/100 mg tissue; and 16.63, 9.32, 31.92 pg/100 mg tissue, respectively.
Variations in placental TNF-α protein expression noted at different trimesters may suggest gestational age specific roles for the cytokine. The increase in TNF-α protein expression observed in the second trimester may be involved in upregulating synthesis of MMP and in augmenting apoptosis of vascular smooth muscle cells of the spiral arteries. A failure in this second trimester increase in TNF-α protein could contribute to gestational compromise.
胎盘肿瘤坏死因子-α（TNF-α）是一种细胞信号蛋白。在孕期，TNF-α诱导基质金属蛋白酶（MMPs）的合成，使细胞滋养层能够深入子宫蜕膜内的螺旋动脉。TNF-α还可增强这些动脉周围血管平滑肌细胞的凋亡。本研究调查了正常人类妊娠期胎盘绒毛中TNF-α蛋白的表达情况。
使用酶免疫分析试剂盒（开曼化学公司，密歇根州安阿伯市，产品编号589201）对因选择性手术终止妊娠和正常足月分娩获得的胎盘绒毛组织进行检测。
孕早期（N = 99）、孕中期（N = 58）和孕晚期（N = 42）的中位数、第25百分位数和第75百分位数分别为：36.46、27.25、45.90 pg/100 mg组织；55.43、40.09、110.88 pg/100 mg组织；以及16.63、9.32、31.92 pg/100 mg组织。
不同孕期胎盘TNF-α蛋白表达的变化可能提示该细胞因子在特定孕周发挥作用。孕中期观察到的TNF-α蛋白表达增加可能参与上调MMP的合成以及增强螺旋动脉血管平滑肌细胞的凋亡。孕中期TNF-α蛋白未能增加可能导致妊娠并发症。
