Chen Jing, Zhang Jianling, Han Buxing, Li Zhonghao, Li Junchun, Feng Xiaoying
Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.
Colloids Surf B Biointerfaces. 2006 Mar 1;48(1):72-6. doi: 10.1016/j.colsurfb.2006.01.010. Epub 2006 Feb 24.
A new method to prepare the cross-linked enzyme aggregates (CLEAs) was developed. Through cross-linking the enzyme (Trypsin) aggregates, which was precipitated from the CO2-expanded reverse micellar solutions, dendritic CLEAs were obtained. The sizes of the CLEAs prepared by this new method were nanometer order of magnitudes and could be tuned by changing the water-to-surfactant ratio (w0) and the concentration of enzyme in the reverse micellar solution. The diameter of CLEAs increased with increasing w0 value of reverse micelles and the concentration of Trypsin. The activity of CLEAs obtained by this method is improved in contrast to those obtained by the conventional method. This method has some advantages in applications and can be easily applied to the synthesis of other cross-linked enzyme aggregates.
开发了一种制备交联酶聚集体（CLEAs）的新方法。通过交联从CO2膨胀反胶束溶液中沉淀出的酶（胰蛋白酶）聚集体，获得了树枝状CLEAs。用这种新方法制备的CLEAs尺寸为纳米量级，可通过改变水与表面活性剂的比例（w0）和反胶束溶液中酶的浓度来调节。CLEAs的直径随着反胶束w0值和胰蛋白酶浓度的增加而增大。与传统方法获得的CLEAs相比，用该方法获得的CLEAs活性有所提高。该方法在应用方面具有一些优势，并且可以很容易地应用于其他交联酶聚集体的合成。
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