交联酶聚集体（CLEAs）的制备、优化及结构
Preparation, optimization, and structures of cross-linked enzyme aggregates (CLEAs).
作者信息
Schoevaart R, Wolbers M W, Golubovic M, Ottens M, Kieboom A P G, van Rantwijk F, van der Wielen L A M, Sheldon R A
机构信息
Biocatalysis and Organic Chemistry, Department of Biotechnology, Delft University of Technology, Julianalaan 136, 2628 BL, The Netherlands.
出版信息
Biotechnol Bioeng. 2004 Sep 20;87(6):754-62. doi: 10.1002/bit.20184.
The broad applicability of the cross-linking of enzyme aggregates to the effective immobilisation of enzymes is demonstrated and the influence of many parameters on the properties of the resulting CLEAs is determined. The relative simplicity of the operation ideally lends itself to high-throughput methodologies. The aggregation method was improved up to 100% activity yield for any enzyme. For the first time, the physical structures of CLEAs are elucidated.
证明了酶聚集体交联在酶有效固定化方面的广泛适用性，并确定了许多参数对所得交联酶聚集体性质的影响。该操作相对简单，非常适合高通量方法。对于任何酶，聚集方法的活性产率提高到了100%。首次阐明了交联酶聚集体的物理结构。
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