Choi H U, Meyer K
Carbohydr Res. 1975 Mar;40(1):77-88. doi: 10.1016/s0008-6215(00)82670-1.
The structure of an antigenic, sulfated glycoprotein from chick chorioallantoic fluid has been investigated by exogalactosidase digestion, methylation and mass spectral analyses, periodate oxidation, and Smith degradation. The main carbohydrate chains are composed of D-galactosyl residues linked at C-3 and 2-acetamido-2-deoxyglucose residues linked at C-4. Fucose and N-acetylneuraminic acid residues are nonreducing terminal groups, and the N-acetylneuraminic acid groups are linked to the D-galactose residues at C-3. Most of the sulfate groups (91% of the sulfate) are located on C-6 of the 2-acetamido-2-deoxyglucose residues, and the rest on C-6 of the D-galactose residues. A large number of the D-galactose residues (36.9% of the total) are present as nonreducing terminal groups and another 21.7% of the D-galactose residues are in penultimate position to the nonreducing terminal N-acetylneuraminic acid residues. Although mild periodate oxidation indicates the presence of D-galactose in furanoside form (5.5% of total D-galactose), no 5-O-methyl derivative of D-galactose was observed on methylation.
通过外切半乳糖苷酶消化、甲基化和质谱分析、高碘酸盐氧化以及史密斯降解法，对鸡胚绒毛尿囊液中的一种抗原性硫酸化糖蛋白的结构进行了研究。主要的碳水化合物链由连接在C-3位的D-半乳糖基残基和连接在C-4位的2-乙酰氨基-2-脱氧葡萄糖残基组成。岩藻糖和N-乙酰神经氨酸残基为非还原末端基团，且N-乙酰神经氨酸基团连接在C-3位的D-半乳糖残基上。大部分硫酸基团（占硫酸总量的91%）位于2-乙酰氨基-2-脱氧葡萄糖残基的C-6位，其余的位于D-半乳糖残基的C-6位。大量的D-半乳糖残基（占总量的36.9%）以非还原末端基团形式存在，另外21.7%的D-半乳糖残基位于非还原末端N-乙酰神经氨酸残基的倒数第二位。尽管温和的高碘酸盐氧化表明存在呋喃糖苷形式的D-半乳糖（占总D-半乳糖的5.5%），但甲基化时未观察到D-半乳糖的5-O-甲基衍生物。
