Harik S I, Roessmann U
Department of Neurology, University Hospital of Cleveland, OH 44106.
Ann Neurol. 1991 May;29(5):487-91. doi: 10.1002/ana.410290507.
We previously demonstrated that brain microvessels have a high density of the erythrocyte-type glucose transporter and suggested this could be used as a marker of cells with "occluding" junctions. Now, we have studied by immunocytochemistry the tissue distribution of the glucose transporter in a variety of primary and metastatic human brain tumors with a monoclonal antibody to the glucose transporter of human erythrocytes. Microvessels in normal brain tissue distant from the tumor, and in brain surrounding the tumor, immunostained strongly for the glucose transporter. Microvessels in primary and metastatic tumors, however, lacked glucose transporter immunoreactivity. Only in the relatively benign juvenile cerebellar astrocytomas was glucose transporter immunoreactivity retained in some of the tumor microvessels. Because a high glucose transporter density is a marker of vessels with barrier properties, our results indicate that the vast majority of brain tumor microvessels do nc, have an intact blood-brain barrier.
我们先前证明脑微血管具有高密度的红细胞型葡萄糖转运体，并提出这可作为具有“封闭”连接的细胞的标志物。现在，我们用抗人红细胞葡萄糖转运体的单克隆抗体，通过免疫细胞化学研究了葡萄糖转运体在多种原发性和转移性人脑肿瘤中的组织分布。远离肿瘤的正常脑组织以及肿瘤周围脑组织中的微血管，对葡萄糖转运体呈强免疫染色。然而，原发性和转移性肿瘤中的微血管缺乏葡萄糖转运体免疫反应性。仅在相对良性的青少年小脑星形细胞瘤中，部分肿瘤微血管保留了葡萄糖转运体免疫反应性。由于高葡萄糖转运体密度是具有屏障特性血管的标志物，我们的结果表明绝大多数脑肿瘤微血管没有完整的血脑屏障。
