Zhang M, Olsson Y
Laboratory of Neuropathology, University Hospital, Uppsala, Sweden.
APMIS. 1996 Apr;104(4):293-301.
The expression of the glucose transporter protein, GLUT 1, in endothelial cells of microvessels within and around hematogenous metastases of the human brain was investigated by immunohistochemistry using a polyclonal antibody raised against the carboxyl terminus of the transporter molecule. The metastases were obtained from 18 autopsy cases with pulmonary carcinomas. Endothelial cells of controls without evidence of brain pathology showed a strong immunoreactivity, indicating that the antigenic sites of the glucose transporter remained in the postmortem material. The endothelial cells of microvessels around the metastases did not show any changes with regard to expression of the glucose transporter. However, in 14 of the 18 tumor cases, microvessels located in the metastases did not express the transporter. Our results indicate that in human cases of brain metastases functional changes with regard to glucose transport occur within the metastases rather than in the peritumoral region.
利用针对转运分子羧基末端产生的多克隆抗体，通过免疫组织化学法研究了人脑血行转移灶内部及周围微血管内皮细胞中葡萄糖转运蛋白GLUT 1的表达情况。这些转移灶取自18例肺癌尸检病例。无脑部病变证据的对照者的内皮细胞显示出强免疫反应性，表明葡萄糖转运蛋白的抗原位点在尸检材料中依然存在。转移灶周围微血管的内皮细胞在葡萄糖转运蛋白表达方面未显示任何变化。然而，在18例肿瘤病例中的14例中，位于转移灶内的微血管未表达该转运蛋白。我们的结果表明，在人脑转移病例中，葡萄糖转运方面的功能变化发生在转移灶内而非肿瘤周围区域。
