Gabrielle L, Leterrier F, Cristau P, Laverdant C
Clin Chim Acta. 1975 Apr 16;60(2):147-55. doi: 10.1016/0009-8981(75)90120-5.
Human liver cytochrome P-450 level has beenen determined by direct electron paramagnetic resonance spectroscopy at 77 degrees K of liver specimens obtained by surgical biopsy. A preliminary experiment with rat livers was performed in order to calibrate the intensity of the cytochrome P-450 EPR signal as a function of the cytochrome concentration obtained by the classical spectrophotometric method. 44 subjects have be examined. We found a cytochrome P-450 value of 21.5 plus or minus 11.2 nmoles per gram of liver in normal subjects. Variations of this level in some pathological liver states are discussed.
已通过对手术活检获取的肝脏标本在77开尔文温度下进行直接电子顺磁共振光谱法测定了人肝脏细胞色素P - 450水平。为了将细胞色素P - 450电子顺磁共振信号强度校准为经典分光光度法测得的细胞色素浓度的函数，对大鼠肝脏进行了初步实验。已检查了44名受试者。我们发现正常受试者每克肝脏的细胞色素P - 450值为21.5±11.2纳摩尔。讨论了某些病理性肝脏状态下该水平的变化。
