Kratz F
Eur J Clin Pharmacol. 1976 Jun 15;10(2):133-7. doi: 10.1007/BF00609472.
The specific activity of coumarin-7-hydroxylase was measured in liver microsomes from normal subjects and patients with liver disease. Liver specimens were obtained by needle biopsy and the microsomal fraction was separated by differential centrifugation. Its freedom from mitochondria was demonstrated by the absence of succinic dehydrogenase, a marker enzyme for mitochondria. Liver from healthy subjects showed variation in the specific activity of coumarin-7-hydroxylase from 0.16 to 0.65 nmol-mg-1-min-1, which is probably due to genetic factors. Patients with cirrhosis of the liver, chronic fatty hepatitis (chronic alcoholic hepatitis) and chronic active hepatitis showed a significantly lower mean hydroxylase activity. There was no significant difference in the mean level of hydroxylase between patients with subacute viral hepatitis or chronic persistent hepatitis and the normal controls.
在正常受试者和肝病患者的肝微粒体中测定了香豆素-7-羟化酶的比活性。通过针吸活检获取肝脏标本，采用差速离心法分离微粒体部分。通过缺乏琥珀酸脱氢酶（一种线粒体标记酶）证明其不含线粒体。健康受试者肝脏中香豆素-7-羟化酶的比活性在0.16至0.65 nmol·mg⁻¹·min⁻¹之间变化，这可能是由遗传因素导致的。肝硬化、慢性脂肪性肝炎（慢性酒精性肝炎）和慢性活动性肝炎患者的平均羟化酶活性显著降低。亚急性病毒性肝炎或慢性持续性肝炎患者与正常对照组之间的羟化酶平均水平无显著差异。
