Knecht H, Odermatt B F, Bachmann E, Teixeira S, Sahli R, Hayoz D, Heitz P, Bachmann F
Department of Medicine, University Hospital CHUV Lausanne, Switzerland.
Blood. 1991 Aug 1;78(3):760-7.
This study of 52 Swiss patients with Hodgkin's disease (HD), including 17 cases with a high content of Sternberg-Reed (SR) and Hodgkin (H) cells, was performed to determine the percentage of cases harboring Epstein-Barr virus (EBV) DNA and/or clonal rearrangements of Ig and T-cell antigen receptor (TcR) genes in diagnostic lymph node biopsies. Special attention was drawn to the heavily infiltrated cases to detect a possible relationship between clonality and EBV DNA identification. EBV DNA was detected by the polymerase chain reaction (PCR) using three different sets of specific primers. The viral origin of the amplification products was confirmed by hybridization with a radiolabeled internal probe or demonstration of a specific Sma I restriction site. Genomic rearrangement of Ig and TcR genes was studied by Southern blot analysis. EBV DNA was identified by PCR in 38 of 48 cases (79%). Clonal rearrangements were identified in only 4 of 52 cases (Ig genes) and were independent of the degree of infiltration by SR cells and the presence of EBV DNA. The absence of EBV DNA in three cases with numerous SR cells (only one of them showed clonal rearrangement) and the presence of only a few viral copies in four further cases with numerous SR cells (semiquantitative analysis of viral DNA by PCR was performed in 26 EBV-positive cases) suggests that this virus is modulating rather than an etiologic agent in a considerable proportion of HD cases.
本研究对52例瑞士霍奇金淋巴瘤（HD）患者进行，其中包括17例含有大量施特恩贝格-里德（SR）细胞和霍奇金（H）细胞的病例，旨在确定诊断性淋巴结活检中携带爱泼斯坦-巴尔病毒（EBV）DNA和/或免疫球蛋白（Ig）及T细胞抗原受体（TcR）基因克隆重排的病例百分比。特别关注重度浸润病例，以检测克隆性与EBV DNA鉴定之间的可能关系。使用三组不同的特异性引物通过聚合酶链反应（PCR）检测EBV DNA。扩增产物的病毒来源通过与放射性标记的内部探针杂交或特定Sma I限制性位点的证明来确认。通过Southern印迹分析研究Ig和TcR基因的基因组重排。48例中有38例（79%）通过PCR鉴定出EBV DNA。仅在52例中的4例（Ig基因）中鉴定出克隆重排，且与SR细胞的浸润程度和EBV DNA的存在无关。3例有大量SR细胞的病例中未检测到EBV DNA（其中仅1例显示克隆重排），另外4例有大量SR细胞的病例中仅检测到少量病毒拷贝（对26例EBV阳性病例进行了病毒DNA的半定量PCR分析），这表明在相当一部分HD病例中，这种病毒起调节作用而非病因作用。
