Herbst H, Dallenbach F, Hummel M, Niedobitek G, Pileri S, Müller-Lantzsch N, Stein H
Institute of Pathology, Klinikum Steglitz, Free University of Berlin, Federal Republic of Germany.
Proc Natl Acad Sci U S A. 1991 Jun 1;88(11):4766-70. doi: 10.1073/pnas.88.11.4766.
Cryostat sections from lymph nodes of 47 Hodgkin disease patients were examined by immunohistology for the Epstein-Barr virus (EBV)-encoded latent membrane protein (LMP), nuclear antigen 2, and late viral glycoprotein gp350/250. A distinct LMP-specific membrane and cytoplasmic staining was detected exclusively in Hodgkin and Reed-Sternberg cells in 18 patients (38%); EBV nuclear antigen 2 and gp350/250 immunoreactivity was absent in all instances. Thirty-two of 47 (68%) cases contained EBV-specific DNA sequences as detected by PCR, all LMP-positive cases being in this category. Our results confirm previous studies establishing the presence of EBV genomes in Hodgkin and Reed-Sternberg cells by demonstrating expression of an EBV-encoded protein in the tumor-cell population. The finding of LMP expression in the absence of EBV nuclear antigen 2 suggests a pattern of EBV gene expression different from that of B-lymphoblastoid cell lines and Burkitt lymphoma, whereas this finding shows similarities with that seen in undifferentiated nasopharyngeal carcinoma. Because the LMP gene has transforming potential, our findings support the concept of a pathoetiological role of EBV in many cases of Hodgkin disease.
对47例霍奇金病患者淋巴结的低温切片进行免疫组织学检查，检测爱泼斯坦-巴尔病毒（EBV）编码的潜伏膜蛋白（LMP）、核抗原2和晚期病毒糖蛋白gp350/250。仅在18例患者（38%）的霍奇金细胞和里德-斯腾伯格细胞中检测到明显的LMP特异性膜和细胞质染色；所有病例均未检测到EBV核抗原2和gp350/250免疫反应性。47例中有32例（68%）通过聚合酶链反应检测到EBV特异性DNA序列，所有LMP阳性病例均在此类别中。我们的结果通过证实在肿瘤细胞群体中表达一种EBV编码蛋白，证实了先前关于霍奇金细胞和里德-斯腾伯格细胞中存在EBV基因组的研究。在缺乏EBV核抗原2的情况下发现LMP表达，提示EBV基因表达模式不同于B淋巴母细胞系和伯基特淋巴瘤，而这一发现与未分化鼻咽癌的情况相似。由于LMP基因具有转化潜能，我们的发现支持EBV在许多霍奇金病病例中具有致病作用的概念。
