Bignon Y J, Bernard D, Curé H, Fonck Y, Pauchard J, Travade P, Legros M, Dastugue B, Plagne R
Laboratoire d'Oncologie Moléculaire, Centre Jean Perrin, Clermont-Ferrand, France.
Mol Carcinog. 1990;3(1):9-11. doi: 10.1002/mc.2940030105.
Previous studies using Southern blot analysis or in situ hybridization have shown that approximately 20% of patients with Hodgkin's disease have Epstein-Barr virus (EBV) in involved tissues. We used the more sensitive polymerase chain reaction (PCR) technique to determine if a higher percentage of EBV could be detected. Of the 16 Hodgkin's disease patients studied, the PCR technique detected EBV in eight (50%). No prognostic significance was associated with the presence of EBV in the eight EBV-positive patients, and the presence of EBV was not associated with B cell monoclonality.
以往使用Southern印迹分析或原位杂交的研究表明，约20%的霍奇金病患者受累组织中存在爱泼斯坦-巴尔病毒（EBV）。我们使用更敏感的聚合酶链反应（PCR）技术来确定是否能检测到更高比例的EBV。在研究的16例霍奇金病患者中，PCR技术在8例（50%）患者中检测到了EBV。8例EBV阳性患者中EBV的存在与预后无关，且EBV的存在与B细胞单克隆性无关。
