通过表面增强拉曼散射光谱监测枯草芽孢杆菌芽孢萌发的动力学。

Monitoring the kinetics of Bacillus subtilis endospore germination via surface-enhanced Raman scattering spectroscopy.

作者信息

Daniels Jacquitta K, Caldwell Thomas P, Christensen Kenneth A, Chumanov George

机构信息

Department of Chemistry, Clemson University, Clemson, South Carolina 29634, USA.

出版信息

Anal Chem. 2006 Mar 1;78(5):1724-9. doi: 10.1021/ac052009v.

DOI:10.1021/ac052009v

PMID:16503629

Abstract

Mirror sandwich SERS substrates (M3S) were used to monitor the kinetics of Bacillus subtilis endospore germination. The sandwich configuration of the substrates allows real-time observation of germination in samples that contained only several hundred endospores. The enhancement provided by the substrates is attributed to the enhanced local electromagnetic field that originates from coupling between the Ag nanoparticles and the underlying metal film as well as from coupling between the Ag nanoparticles themselves. The germination kinetics at varying concentrations of l-alanine and different temperatures were studied by monitoring the intensity and growth of the Raman peak at 1010 cm(-1), which is characteristic of dipicolinic acid. A total of four concentrations (50, 75, 100, and 150 mM) of l-alanine and three different temperatures (30, 37, and 55 degrees C) were investigated.

摘要

镜像夹心表面增强拉曼散射基底（M3S）被用于监测枯草芽孢杆菌芽孢萌发的动力学过程。基底的夹心结构能够实时观察仅含有几百个芽孢的样品中的萌发情况。基底所提供的增强作用归因于银纳米颗粒与下层金属膜之间的耦合以及银纳米颗粒自身之间的耦合所产生的增强局部电磁场。通过监测1010 cm⁻¹处吡啶二羧酸特征拉曼峰的强度和增长情况，研究了不同浓度的L-丙氨酸和不同温度下的萌发动力学。共研究了四种浓度（50、75、100和150 mM）的L-丙氨酸以及三种不同温度（30、37和55℃）。