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通过表面增强拉曼散射显微镜监测单个枯草芽孢杆菌芽孢萌发过程中的二吡啶胺释放。

Monitoring DPA release from a single germinating Bacillus subtilis endospore via surface-enhanced Raman scattering microscopy.

作者信息

Evanoff David D, Heckel John, Caldwell Thomas P, Christensen Kenneth A, Chumanov George

机构信息

Department of Chemistry, Clemson University, Clemson, SC 29634, USA.

出版信息

J Am Chem Soc. 2006 Oct 4;128(39):12618-9. doi: 10.1021/ja0642717.

DOI:10.1021/ja0642717

PMID:17002334

Abstract

A method for monitoring DPA release from a single germinating Bacillus subtilis endospore is reported. High S/N ratio SERS spectra were obtained with excitation power 3 mW at 647.1 nm and 1 min spectral collection times. The method is proof-of-principle for the SERS detection limit at the single spore level. This represents a 100- to 1000-fold improvement over previously reported detection limits for SERS-based measurements of DPA in endospores.

摘要

报道了一种监测单个发芽枯草芽孢杆菌芽孢中吡啶二羧酸（DPA）释放的方法。在647.1nm激发波长下，激发功率为3mW，光谱采集时间为1分钟，获得了高信噪比的表面增强拉曼散射（SERS）光谱。该方法证明了在单芽孢水平上SERS检测限的原理。这比之前报道的基于SERS测量芽孢中DPA的检测限提高了100至1000倍。